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| Bruno,J.B.; Lima-Verde,I.B.; Martins,F.S.; Matos,M.H.T.; Lopes,C.A.P.; Maia-Jr.,J.E.; Báo,S.N.; Nobre Junior,H.V.; Maia,F.D.; Pessoa,C.; Moraes,M.O.; Silva,J.R.V.; Figueiredo,J.R.; Rodrigues,A.P.R.. |
| Avaliou-se o efeito da adição de diferentes tipos e concentrações de soro sobre o desenvolvimento e a sobrevivência de folículos ovarianos pré-antrais (FOPA) caprinos in vitro. Além disso, verificou-se a relação entre as concentrações de nitrito presentes no meio de cultivo e a viabilidade folicular. Cada par ovariano foi dividido em 29 fragmentos, sendo um destinado ao controle. Os fragmentos foram cultivados por um ou sete dias em meio essencial mínimo suplementado (MEM+) ou MEM+ com diferentes concentrações (10 ou 20%) de soro fetal bovino (SFB), soro de cabra em estro (SCE) ou soro de cabra em diestro (SCD). Na análise morfológica após sete dias, apenas o tratamento com 10% de SFB apresentou percentual de FOPA normais similar ao MEM+ (P>0,05). A... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Caprino; Folículo pré-antral; Cultivo in vitro; Soro. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000600006 |
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| Martins,F.S.; Celestino,J.J.H.; Saraiva,M.V.A.; Chaves,R.N.; Rossetto,R.; Silva,C.M.G.; Lima-Verde,I.B.; Lopes,C.A.P.; Campello,C.C.; Figueiredo,J.R.. |
| The objective of this study was to determine the effects of GDF-9, IGF-I, and GH alone or combined on preantral follicle survival, activation and development after 1 and 7 days of in vitro culture. Either fresh (non-cultured) or cultured ovarian tissue was processed for histological and fluorescence analysis. For all media tested, the percent of normal follicles was greater when compared to minimum essential medium supplemented (MEM+) alone, except when ovarian tissue was cultured with GDF-9/IGF-I or GDF-9/GH (P < 0.05). Fluorescence analysis showed that the percent of viable follicles after 7 days of culture was similar for non-cultured tissue and for all treatments tested. The percent of primordial follicles was reduced (P < 0.05) and there was a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GDF-9; IGF-I; GH; Culture; Follicle activation; Goat. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800006 |
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| Rodrigues,G.Q.; Lima,I.M.T.; Chaves,R.N.; Rossetto,R.; Costa,S.L.; Castro,S.V.; Barros,V.R.P.; Matos,M.H.T.; Lopes,C.A.P.; Báo,S.N.; Campello,C.C.; Figueiredo,J.R.. |
| The objectives of this study were to investigate whether TGF-β affect the survival, activation and further growth of goat primordial follicles enclosed in ovarian cortex after in vitro culture. Goat ovaries were collected from an abattoir and pieces of ovarian tissues were cultured for one or seven days in a supplemented alpha Minimum Essential Medium, alone or containing TGF-β (1, 5, 10 or 50ng/mL). Ovarian tissues from the fresh control as well as those cultured were processed for histological and ultrastructural studies. The results showed that when compared with fresh control, there was decrease in the percentages of histologically normal follicles in all treatments only after seven days culture. TGF-β did not affect the activation of preantral... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Preantral follicle; In vitro development; TGF-β. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000200013 |
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